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Cardiovascular and Renal Research

Research areas

  • Renal and cardiovascular regulation of salt and water balance and blood pressure in the normal condition, during development, and under pathomogical salt and water retaining conditions
  • Cardiac stem cells
  • Diabetes-associated vascular changes
  • Receptor pharmacology and Cellular mechano-electrical transduction

Available methodologies, techniques and facilities

  • Cell isolation and cell culture facilities for cell lines and primary cultures (juxtaglome-rular cells, vascular smooth muscle (renal, rat and human aortic), inner medullary collecting duct cells, mesangial cells, renin-producing AS4.1 cells, human aortic endothelial cells, rat cardiac myocytes and fibroblasts (neonatal and adult), and cardiac stem cells.
  • Conscious dog and rat setup with servo mechanisms controlling body contents of water and salt and blood pressure.
  • On line recordings of blood pressure in conscious unstressed mice
  • Electrophysiological recordings on single cells (tight seal whole cell recordings): membrane potential (current clamp), current-voltage relationship, single channel (voltage clamp) and membrane capacitance.
  • Using chamber with determination of transepithelial resistance and currentof epithelial monolayers.
  • ELISA
  • MicroRNA detection and visualization methods, including quantitative RT-PCR and in situ hybridization.
  • Flow cytometry and cell sorting (FACS) of primary isolated cells (at the FACS Core facilities).
  • Culturing and differentiation of murine embryonic stem cells.
  • Quantification and profiling of cell numbers and cell sizes by use of Coulter counter (Z2).
  • Mice models of cardiac diseases, including aortic stenosis and induction of cardiac infarctions.
  • Enzyme activity measurements (nitric oxide synthase).
  • Immunohistochemistry, immunohisto- and –cyto fluorescense for enzymes, ion transporters, proliferation, growth factors, hypoxia, and cell type specific markers..
  • Intracellular pH and calcium concentration measurements by ratio fluorescence spectrometry and laser confocal imaging.
  • Ion currents in isolated sensory epithelia by the voltage clamp technique.
  • Measurement of metabolites (column chromatography; HPLC).
  • Microdissection of blood vessels and single nephron segments
  • Microperfusion of arteries and small blood vessels
  • Recordings of isometric force in artery rings from rat, rabbit, mouse
  • Microspectrofluorimetry.
  • Molecular biology including isolation of RNA and DNA from cells and tissues, cDNA cloning, sequencing, RT-PCR, RNase protection assay, RNA differential display, Northern and Southern blotting, Western blotting, quantitative real-time PCR with SYBR green detection, transfection, site-directed mutagenesis, siRNA silencing.
  • Osmotic pressure measurements.
  • Plasma volume determination.
  • Radioimmunoassays of vasoactive hormones, prostaglandins and cyclic nucleotides.
  • Rat model for liver cirrhosis and nephrotic syndrome and adrenal insufficiency (adrenalectomy).
  • Mouse trangenic breeding (eNOS-/-, COX-2-/-).
  • Rat model for renal ischemia-reperfusion and infarct.
  • Determination of urine concentration ability in rats and tissue interstitial osmolality.
  • Receptor subtype determination.
  • Renal Clearance techniques.
  • Renin secretion from superfused and incubated juxtaglomerular cells.
  • Determination of isometric force generation in arteries from mice, rats, and humans by myograph technique.
  • Tension-measurements in smooth muscle.
  • Uptake and release of transmitters determined by HPLC and liquid scintillation spectrometry.
  • Available methodologies, techniques and facilities

Cardiovascular and Renal Research is a research unit of Department of Molecular Medicine.

 Head of research professor Boye L. Jensen

 

 

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