- Development of novel hybridoma derived antibodies
- Characterization of protein-protein interactions using SPR, BLI and GCI
- Recombinant protein production, purification and engineering: Engineering includes design of fusion proteins, and bispecifics, bifunctional, fragments/ScFv and point mutations, such as Fc-neutralizing point mutations.
- Synthesis of labelled antibodies including antibody drug conjugates
- Assay development; AlphaLiza, ELISA, Luminex
- Animal and cellular models
- RT-qPCR
- Isolation of various cell types (e.g. epithelial cells from intestine and kidney, lamina propria lymphocytes from small and large intestine, leukocytes from lymph nodes).
- Flow cytometry analysis and FACS sorting
Generation of human and mouse-derived conventional and apical-out intestinal organoids - Immunofluorescence staining and imaging of frozen and paraffin embedded sections Isolation of fecal DNA from mice and man – assays for the detection of commensal protozoa, fungi and bacteria species.
Specific apparatus
- Surface plasmon resonance (SPR): Biacore T200, Cytiva
- Grating Coupled interferometry (GCI): Creoptix WAVEdelta, Malvern Panalytical
- Biolayer interferometry(BLI): Octet RED96e, Satorius
- Dynamic light Scattering (DLS): Zetasize Advance Ultra (red)
- Automated capillary western blotting: Jess, ProteinSimple
Specific assays and diseasemodels
- Surfactant protein D (SP-D, lung injury marker) in mouse and man
- Microfibrillar-associated Protein 4 (MFAP4, liver fibrosis marker) in mouse and man
- Fibrinogen-related domain containing 1 (FIBCD1, soluble form, myokine) in mouse and man
- Cell assays; Cell migration (scratch assay, transwell, oris assay), adhesion (incl focal adhesion), proliferation, inflammation response, uptake assays
- Various mice models (atherosclerosis, abdominal aneurysm formation, kidney fibrosis, liver fibrosis, cigarette smoke exposure, chronic intestinal inflammation, MASH and cancer). Invasive lung function measurements in mice and airway exposure of aerosols.
