Immunological methods
- Multicolor flow cytometry (3 lasers, 9 colors)
- ELISA
- Mixed lymphocyte culture
- Functional T cell assay
- T cell activation assay
- Functional CD8+ T cell assay
- Cytotoxic lymphocyte assays
- NK cell assays
- Immunohistochemistry (in collaboration with Pathology)
- Immunofluorescence
- Western blotting
- xMAP technology (Luminex)
- Determination of HLA types
- Complement-dependent microtoxicity assays
Cell biology methods
- Cell culture of human primary cells and cell lines
- Isolation of peripheral blood mononuclear cells
- Isolation of mesenchymal stem cells from bone marrow aspirates (in collaboration with others)
- Mycoplasma analysis
- Cell culture in hypoxia chamber
- Bacterial cell cultures
- Human cell transfection (liposome-based and electroporation)
- Isolation of single-cell clones for stable transfections
- Phase contrast and fluorescence microscopy
- Cryogenic storage of cells
- Senescence-specific staining analysis
- Gamma-ray irradiation of cells
- Magnetic bead conjugates for cell-type-specific isolations
- Magnetic bead conjugates for mitochondria isolations
Molecular biology methods
- Standard PCR
- Real-time PCR
- Isolation of DNA from human cells and virus
- Isolation of RNA from human cells and virus
- cDNA synthesis
- Preparation of protein extracts
- DNA sequencing
- DNA fragment analysis
- Gel electrophoresis (DNA and proteins)
- Isolation of plasmid DNA
- Cloning of DNA fragments
- Construction of expression vectors
- Nanodrop spectrophotometry
- Fluorometry
Other (soon to be established)
- Autoradiography (estimation of proliferation index)
- BrDU-incorporation assays (estimation of proliferation index)
- Luciferase-based expression systems
- Isolation of microRNA
- Cloning of microRNA