Research interest
The research group is closely associated with the Odense University Hospital and performs research at the translational level within two main areas:
Chronic kidney injury and mechanisms of renal fibrosis
Renal fibrosis is the final common pathway in all chronic kidney diseases and is associated with loss of kidney function. Our research group focuses on elucidating novel mechanisms of renal fibrogenesis with the aim of identifying new targets for renoprotective treatment and to increase basic knowledge of extracellular matrix homeostasis. We collaborate with the Department of Nephrology, Urology and Pathology, Odense University Hospital, and we perform research projects using samples collected from patients diagnosed with chronic kidney disease, human kidney tissue, animal models of chronic kidney injury and cell culture experiments.
Tyrosine kinase inhibitor-induced hypertension
Tyrosine kinase inhibitors (TKIs) are new drugs used in the medical treatment of several cancer diseases. A common side effect is hypertension due to an unknown mechanism. Our research group focuses on elucidating mechanisms of TKI-induced hypertension with the aim of improving anti-hypertensive treatment in this group of patients and to increase basic knowledge of blood pressure regulation. We collaborate with the Department of Oncology, Odense University Hospital, and we perform research projects using samples collected from TKI-treated patients, human kidney tissue, animal models of TKI-induced hypertension and cell culture experiments.
Methods and Techniques
In vivo studies of kidney development in mice and rats
Blood pressure measurements in rats
Chronic kidney injury models in mice and rats
Transgenic animal models
Blood pressure measurements, plasma and urinary analyses in patients
Ex vivo slice culture of human kidney tissue
Analyses of human kidney tissue
Cell culture models
Stereology
RT-PCR and quantitative PCR
Western blotting, immunohistochemistry and immunofluorescence
ELISA and RIA
Staff
Laboratory technician Amalie Kamstrup Mogensen
Courses
Bachelor module B5 ”Heart, Circulation and Respiration” (Medicine and Biomechanics)
Human Pathophysiology (Science)
Bachelor module B12 “From Health to Sick” (Medicine and Biomechanics)
Renal pathology, specialist training (hoveduddannelse) in forensic medicine
Renal developmental pathology, specialist training (hoveduddannelse) in nephrology
Animal research in experimental pathology, specialist training (hoveduddannelse) in pathology
Collaborations
Consultant Lars Bastholt, Department of Oncology, Odense University Hospital
Consultant Claus Bistrup, Department of Nephrology, Odense University Hospital
Professor Niels Marcussen, Department of Pathology, Odense University Hospital
Associate Professor Rikke Nørregaard, Institute of Clinical Medicine, Aarhus University
Professor Thomas M. Coffman, Division of Nephrology, Duke University, USA
Vladimir T. Todorov, Head of Experimental Nephrology, University of Dresden, Germany
Selected publications
Nilsson L, Madsen K, Krag S, Frøkiær J, Jensen BL, Nørregaard R. Disruption of cyclooxygenase type 2 exacerbates apoptosis and renal damage during obstructive nephropathy. Am J Physiol Renal Physiol [Epub ahead of print]
Tinning AR, Jensen BL, Schweda F, Muchara K, Hansen PB, Stubbe J, Gramsbergen J, Madsen K. The water channel aquaporin-1 contributes to renin cell recruitment during chronic stimulation of renin production. Am J Physiol Renal Physiol 307:F1215-F1226,2014
Madsen K, Reddy RN, Price SR, Williams CR, Gooch JL. Nutritional intervention restores muscle but not kidney phenotypes in adult calcineurin A-alpha null mice. PLoS One 8:e62503,2013
Madsen K, Tinning AR, Marcussen N, Jensen BL. Postnatal development of the renal medulla; role of the renin-angiotensin system. Acta Physiol Scan 208:41-49,2013
Kjærsgaard G, Madsen K, Marcussen N, Christensen S, Walter S, Jensen BL. Tissue injury after lithium treatment in human and rat postnatal kidney involves glycogen synthase kinase-3beta positive epithelium. Am J Physiol Renal Physiol 302:F455-F465,2012
Nørregaard R, Madsen K, Hansen PB, Bie P, Thalingam S, Frøkiær J, Jensen BL. COX-2 disruption leads to increased central vasopressin stores in and impaired urinary concentrating ability in mice. Am J Physiol Renal Physiol 301:F1303-F1313,2011
Madsen K, Marcussen N, Pedersen M, Kjærsgaard G, Facemire C, Coffman TM, Jensen BL. Angiotensin II promotes development of the renal microcirculation through AT1 receptors. J Am Soc Nephrol 21:448-459,2010
Madsen K, Friis UG, Gooch JL, Hansen PB, Holmgaard L, Skøtt O, Jensen BL. Inhition of calcineurin phosphatase promotes exocytosis of renin from juxtaglomerular cells. Kidney Int 77:110-117,2010