Our research focuses on the development and applications of mass spectrometry-based lipidomics and proteomics technologies for systems biology studies of lipid metabolism and its regulation in health and disease. We use state-of-the-art mass spectrometry technology, design integrated proteolipidomics workflows and execute functional studies to characterize the molecular underpinning of lipid homeostasis in a variety of model organisms.
Head of research: Associate professor, PhD Christer S. Ejsing
Researchers and research group: Christer S. Ejsing Lab
Group webpage: msLipidomics.info
A complete list of publications by Christer S. Ejsing can be found here.
Current research projects
We are continuously looking for motivated students to help us carry out research projects. We offer a wide range of interdisciplinary projects spanning analytical chemistry, lipid biochemistry, systems biology, and computational statistics and data analysis.
Developments of lipidomics workflows
We are continuously expanding the capabilities of our lipidomics platform to support ongoing biological and biomedical projects. Our efforts include developing and validating novel workflows using our state-of-the-art Orbitrap Fusion mass spectrometer. This novel machine is equipped with three mass analyzers, two collision cells and two detectors. This provides new analytical dimensions for structural characterization of lipid molecules and their sensitive and accurate quantification. In addition, we develop methods using a custom-designed liquid chromatography system supporting both normal-phase and reverse-phase separation of lipid molecules.
Development of proteolipidomics technology and systems biology studies of lipid metabolism
We have recently established a systems biology approach capitalizing on quantitative proteomics and lipidomics. We use this technology for systems biology studies of lipid metabolism in the yeast Saccharomyces cerevisiae and in mice. This technology can be extended to functional studies in other organisms (including bacteria).
Other project activities
We are continuously engaged in a variety of collaborative projects with national and international partners. Collaborative projects include:
- Development of pharmacokinetic assays for monitoring drug efficacy (collaborative project with Trond Ulven (IFK)).
- Lipid metabolic studies using mice and the worm Caenorhabditis elegans (project with Nils J. Færgeman (BMB)).
- Sterol metabolism in mammalian cells, functional studies using fluorescence microscopy and lipidomics (project with Danial Wüstner (BMB)).
- Characterization the lipidome of neural stem cells (collaborative project with Morten Meyer (OUH)).
- Molecular characterization of the sperm lipidome and its link to reproductive health (project with the fertility clinic at OUH).
Quantitative analysis of proteome and lipidome dynamics reveals functional regulation of global lipid metabolism.
Casanovas A, Sprenger RR, Tarasov K, Ruckerbauer DE, Hannibal-Bach HK, Zanghellini J, Jensen ON, Ejsing CS.
Chem Biol. 2015 22(3):412-25.
Quantitative spatial analysis of the mouse brain lipidome by pressurized liquid extraction surface analysis.
Almeida R, Berzina Z, Arnspang EC, Baumgart J, Vogt J, Nitsch R, Ejsing CS.Anal Chem. 2015 87(3):1749-56.
Comprehensive lipidome analysis by shotgun lipidomics on a hybrid quadrupole-orbitrap-linear ion trap mass spectrometer.
Almeida R, Pauling JK, Sokol E, Hannibal-Bach HK, Ejsing CS.J Am Soc Mass Spectrom. 2015 26(1):133-48.
The GARP complex is required for cellular sphingolipid homeostasis.
Fröhlich F, Petit C, Kory N, Christiano R, Hannibal-Bach HK, Graham M, Liu X, Ejsing CS, Farese RV, Walther TC.Elife. 2015 10.7554/eLife.08712.
Transformation-associated changes in sphingolipid metabolism sensitize cells to lysosomal cell death induced by inhibitors of acid sphingomyelinase.
Petersen NH, Olsen OD, Groth-Pedersen L, Ellegaard AM, Bilgin M, Redmer S, Ostenfeld MS, Ulanet D, Dovmark TH, Lønborg A, Vindeløv SD, Hanahan D, Arenz C, Ejsing CS, Kirkegaard T, Rohde M, Nylandsted J, Jäättelä M. Cancer Cell. 2013 24(3):379-93.
Orm family proteins mediate sphingolipid homeostasis.
Breslow DK, Collins SR, Bodenmiller B, Aebersold R, Simons K, Shevchenko A, Ejsing CS, Weissman JS. Nature. 2010 463(7284):1048-53.