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Methods & techniques

In vivo methods (mice/rats)

  • Cardiovascular disease models (myocardial infarction, atherosclerosis, aortic aneurysm, vascular injury)
  • Renal disease
  • Adeno-associated virus (AAV)-mediated gene delivery
  • Metabolic balance studies
  • Blood pressure measurements
  • Microsurgery
  • Ultrasound/echocardiography

In vivo methods (human)

  • Insulin clamps
  • Microdialysis
  • Tissue biopsies
  • Arterial and venous catheterization
  • Ultrasound 
  • Metabolic studies
  • Interventional studies (acute and long-term)
  • Exercise interventions

Ex vivo methods

  • Microdissection
  • Wire and pressure myography
  • Microperfusion
  • Human artery biobank
  • Human kidney biobank
  • Kidney tubule preparations

In vitro methods

  • Cell culture
  • Cell transfection
  • Functional assays
  • Electrophysiology

Molecular Biology and Biochemistry

  • Plasmid cloning techniques
  • PCR/qPCR/ddPCR
  • Western blotting
  • Immunohistochemistry/-fluorescence
  • ELISA, RIA
  • HPLC
  • Zymography
  • Histology
  • Bioluminescence
  • Hormone assays (renin, aldosterone, ANGII)
  • Immunoprecipitation
  • cell-surface biotinylation

Imaging

  • Real time NO and ROS measurements
  • Fluorescent imaging
  • Morphometry
  • Confocal laser scanning microscopy 

Last Updated 17.11.2023