Head of the Immunothrombosis Group
Yaseelan Palarasah
Associate professor, PhD
Department of Molecular Medicine
Inflammation Research Unit
Campusvej 55
5230, Odense M
Phone: +45 65504903
ypalarasah@health.sdu.dk
Work/Research Interests
- Innate immunity and inflammation, with a particular emphasis on the complement and contact systems, as well as immunothrombosis.
- The impact of misfolded proteins on the complement and contact systems.
- Detailed bio/immuno-chemical analysis of complement and contact activation to elucidate molecular interactions, activation processes, and the formation of split products
- Translating fundamental immunological discoveries into clinically useful tools.
Methods and Techniques
- Monoclonal antibody production (Hybridoma techniques)
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
- Immunoprecipitation (IP)
- Activated Partial Thromboplastin Time (aPTT) / Kallikrein Generation
- Bio-Plex Multiplex Immunoassay System
- Various assays for complement and contact systems using ELISA and/or Bio-Plex platforms
Courses
- BMB514 - Immunologiske Teknikker for biomedicin- og farmacistuderende
- Krop og folkesundhed
- B10 Angreb og Forsvar
Collaborations
- University of Southern Denmark:
- Assoc. prof. Jonas Heilskov Graversen, Inflammation Research Unit, Department of Molecular Medicine
- Prof. Boye L. Jensen and Prof. Per Svenningsen, Cardiovascular and Renal Research, Department of Molecular Medicine
- The SDU Biomedical Laboratory.
- Odense University Hospital Departments T (Prof. Jes S. Lindholdt), K (Prof. Thomas Emil Andersen), Y (Prof. Claus Bistrup),
- Prof. Claus Nielsen, Principal Scientist, Rigshospitalet, KU
- Prof. Steffen Thiel, Principal Scientist, AU
Membership in Societies
- Member of The International Complement Society
- Scientific and Standardization Committee working group on TG and global hemostasis assays (International Society of Thrombosis and Haemostasis)
Publications
Click here to see all publications
- Palarasah Y, Borg, Bladbjerg EM, Pham STD, Mejldal A, Nielsen C, Pedersen EO, Jensen PB, Thiesson HC, Pilely K. Contact activation system in chronic kidney disease and hemodialysis – A cross-sectional study. Thromb Haemost. 2024 dec 28.
- Isaksson G, Hinrichs G, Andersen A, Bach ML, Weyer K, Zachar R, Henriksen JE, Madsen K, Lund IK, Mollet G, Nielsen M, Bistrup C,Birn H, Jensen BL, Palarasah Y. Amiloride reduces urokinase/plasminogen-driven intratubular complement activation in glomerular proteinuria. J Am Soc Nephrol. 2024 Jan 4.
- Pedersen NB, Bladbjerg EM, Juhl CB, Larsen A, Münster AMB, de Maat A, Palarasah Y. Validation of an ELISA using a new monoclonal antibody specific for the C-terminal fibrinogen γ’ chain: effects of obesity surgery Res Pract Thromb Haemost 2023 Oct 13
- Palarasah Y, Pham STD, Gram JB, Graversen JH, Pilely K, Sidelmann JJ. Plasma kallikrein cleaved H-kininogen: An end-point marker for contact activation in vitro and ex vivo Front Cardiovasc Med. 2022 May 20.
- Palarasah Y, Henriksen ASL, Thiel S, Henriksen M, Hansen SWK. Potency measurements of the complement system facilitated by antibodies targeting the zymogen form of complement factor D (Adipsin). Mol Immunol. 2022 Apr 13.
- Damgaard C, Massarenti L, Danielsen AK, Graversen JH, Holmstrup P, Nielsen CH, Palarasah Y. Complement split product 3 (C3) and its activations split-products in saliva associates with periodontitis J Periodont. 2022 Jan 25.
- Thangaraj SS, Christiansen SH, Graversen JH, Sidelmann JJ, Hansen SWK, Bygum A, Gram JB, Palarasah Y. Contact activation-induced complex formation between complement factor and coagulation factor XIIa. J Thromb Haemost. 2020 Jan 28.
- Biltoft B, Gram JB, Larsen A, Münster AMB, Sidelmann JJ, Skjoedt K, Palarasah Y. Fast form alpha-2-macroglobulin - a marker for protease activation in plasma exposed to artificial surfaces Clin Biochem 2017 Sep
- Rasmussen KJ, Skjoedt MO, Vitved L, Skjoedt K, Palarasah Y. A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product. Immunol Methods. 2017 May
- Pilely K, Skjoedt MO, Nielsen C, Andersen TE, Aabom AL, Vitved L, Koch C, Skjødt K, Palarasah Y. A specific assay for quantification of human C4c by use of an anti-C4c monoclonal antibody. J Immunol Methods. 2014 Jan 25.
Members of the research group
- Jette Hvelplund, Laboratory Technician
- Lars Vitved, Laboratory Technician
- Sai Sindhu Thangaraj, Postdoc
- Stephanie Thuy Doung Pham, PhD student
- Kamilla Bou Færch, Laboratory Technician
