Core Facilities

FACS Core

Click Here to read about FACS Core

MADE

MADE is a national core facility for antibody generation, characterization and development. The core is build on many years of existing expertise in oldest hybridoma technology, long standing experience in ELISA and biosensor assay development and collaboration with a state-of-the-art FACS core facility.

The core offers services in development of antibodies from B-cell derived plasma cells, i.e. antibodies that has been affinity maturated in a mammalian immune system, either in animals or humans. The core facility will offer the following services:

• Design of immunization and screening strategy including production of antigen
• Immunization of animals, access to genetically modified animals
• Classi hybridoma projects in rat and mice
• Functional screening of large number of antigen producing cells (either hybridomas or B-cell derived plasma cells) using droplet fluidics systems, enabling isolation of a very high number of cells producing antibodies binding the antigen
• Antibody sequencing and recombinant expression and purification
• Generation of tailored antibody derivatives, including ADCC enhancement, drug or fluorophore conjugation as well as bispecific antibodies and other antibody fusion proteins
• Extensive characterization of the antibodies using biosensor technology and state of the art FACS equipment
• Advice on development of immunoassays
• Advice on translational development of antibodies

The core will be made available to all biomedical researchers in Denmark in industry and academia.

For projects or questions contact Associate Professor Jonas Heilskov Graversen, jgraversen@health.sdu.dk