Research aim: We aim to develop targeted proteomics workflows for precise and accurate quantitation of proteins and their proteoforms in clinical research and routine.
Projects
- Cardiometabolic protein profiling in large cohorts
- First trimester biomarkers for prediction of gestational diabetes mellitus in obese and non-obese women
- Development of Clinical Applications for Evosep One (Evosep Biosystems, industrial post-doc project)
- A novel targeted multiplex mass spectrometry method for quantitation of abundant matrix and cellular proteins in formalin-fixed paraffin-embedded arterial tissue
Available cohorts and infrastructures
- Stellar linear ion trap mass spectrometer (Thermo Scientific) (part of PLATO)
- Vanquish Flex UPLC (part of PLATO)
- TSQ Altis – triple quadrupole (Thermo Scientific)
- Evosep One integrated HPLC (Evosep Biosystems)
- Opentrons liquid handling robot• Dynex DS2 ELISA Robot
Collaborators
- Odense University Hospital - Lars Melholt Rasmussen, Hans Christian Beck, Dorte Møller Jensen, Kurt Højlund, Thomas Bastholm Olesen, Christina Vinter, Dorte Glintborg, Marianne Skovsager Andersen
- SDU - Lasse Bach Steffensen, Yaseelan Palarasah, Martin Røssel Larsen, Nils Færgeman, Ole Nørregaard Jensen
- Industry/International - Evosep Biosystems, Odense, Richard Kay, MRC Cambridge, UK.
Selected publications
- Faarvang AA, Overgaard M, Jensen PS, Riber LPS, Wod M, Steffensen LB, Rasmussen LM. Arterial collagen IV and laminin levels associate with type 2 diabetes, glycemic control, major adverse cardiac events, and mortality in coronary bypass patients. Cardiovasc Diabetol. 2025 Dec 17;24(1):458.
- Steffensen LB, Larsen JH, Hansen DR, Tha TML, Larsen NS, Beck HC, Rasmussen LM, Overgaard M. Profiling the Plasma Apolipoproteome of Normo- and Hyperlipidemic Mice by Targeted Mass Spectrometry. J Proteome Res. 2023 May 5;22(5):1385-1393.
- Thorsen AF, Riber LPS, Rasmussen LM, Overgaard M. A targeted multiplex mass spectrometry method for quantitation of abundant matrix and cellular proteins in formalin-fixed paraffin embedded arterial tissue. J Proteomics. 2023 Feb 10;272:104775.
- Steffensen LB, Stubbe J, Lindholt JS, Beck HC, Overgaard M, Bloksgaard M, Genovese F, Holm Nielsen S, Tha MLT, Bang-Moeller SK, Hong Lin MKT, Larsen JH, Hansen DR, Jones GT, Bown MJ, Karsdal MA, Rasmussen LM. Basement membrane collagen IV deficiency promotes abdominal aortic aneurysm formation. Sci Rep. (2021) 18;11(1):12903.
- Ravnsborg T, Svaneklink S, Andersen LLT, Larsen MR, Jensen DM, Overgaard M. First-trimester proteomic profiling identifies novel predictors of gestational diabetes mellitus. PLoS One (2019) 27;14(3).
- Ravnsborg T, Andersen LL, Trabjerg ND, Rasmussen LM, Jensen DM, Overgaard M. First-trimester multimarker prediction of gestational diabetes mellitus using targeted mass spectrometry. Diabetologia. (2016) 59(5):970-9.
- Overgaard M, Cangemi C, Jensen ML, Argraves WS, Rasmussen LM. Total and isoform-specific quantitative assessment of circulating fibulin-1 using selected reaction monitoring MS and time-resolved immunofluorometry. Proteomics Clin Appl. (2015) 9(7-8):767-75.