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Methods and techniques

Immunological methods

  • Multicolor flow cytometry (3 lasers, 9 colors)
  • Mixed lymphocyte culture
  • Functional T cell assay
  • T cell activation assay
  • Functional CD8+ T cell assay
  • Cytotoxic lymphocyte assays
  • NK cell assays
  • Immunohistochemistry (in collaboration with Pathology)
  • Immunofluorescence
  • Western blotting
  • xMAP technology (Luminex)
  • Determination of HLA types
  • Complement-dependent microtoxicity assays

Cell biology methods

  • Cell culture of human primary cells and cell lines
  • Isolation of peripheral blood mononuclear cells
  • Isolation of mesenchymal stem cells from bone marrow aspirates (in collaboration with others)
  • Mycoplasma analysis
  • Cell culture in hypoxia chamber
  • Bacterial cell cultures
  • Human cell transfection (liposome-based and electroporation)
  • Isolation of single-cell clones for stable transfections
  • Phase contrast and fluorescence microscopy
  • Cryogenic storage of cells
  • Senescence-specific staining analysis
  • Gamma-ray irradiation of cells
  • Magnetic bead conjugates for cell-type-specific isolations
  • Magnetic bead conjugates for mitochondria isolations

Molecular biology methods

  • Standard PCR
  • Real-time PCR
  • Isolation of DNA from human cells and virus
  • Isolation of RNA from human cells and virus
  • cDNA synthesis
  • Preparation of protein extracts
  • DNA sequencing
  • DNA fragment analysis
  • Gel electrophoresis (DNA and proteins)
  • Isolation of plasmid DNA
  • Cloning of DNA fragments
  • Construction of expression vectors
  • Nanodrop spectrophotometry
  • Fluorometry

Other methods

  • Autoradiography (estimation of proliferation index)
  • BrDU-incorporation assays (estimation of proliferation index)
  • Luciferase-based expression systems
  • Isolation of microRNA
  • Cloning of microRNA


Last Updated 20.10.2023