Research Focus
I investigate the in vivo adipogenic trajectory within epididymal white adipose tissue in mice
Key Findings
- 🧬 Single-nucleus RNA-seq reveals heterogenous subpopulations of adipose stem/progenitor cells
- 📊 Multiome strategy predicts novel signalling pathways and transcription factors relevant for in vivo adipogenesis.
- 🔬 Changes in gene expression and eRegulon modalities observable at high resolution.
Future Plans
I will be working as part of the commercial team in Pentabase, interfacing with distributors and using my expertise in qPCR-based diagnostics assays to provide support to our clients.
Quote
🌟 "Går det, så går det. Og går det ik’, så går det nok alligevel"
Detailed Research Summary
My PhD research focused on the mechanisms underlying in vivo adipogenesis in mouse eWAT. Analyzing combined single-nucleus RNA- and ATAC-seq data, I characterized heterogeneous subpopulations of adipose stem/progenitor cells and placed them along the adipogenic trajectory. The multiomic data allowed me to predict novel ligand–receptor signals and transcription factors associated with adipogenic progression from adipose stem cells to early pre-adipocytes, to late pre-adipocytes, and finally to mature adipocytes. Owing to the high resolution of single-nucleus data, changes in gene expression and enhancer-driven regulatory elements can likewise be observed at high resolution. This atlas provides a one-of-a-kind resource for single-nucleus inquiry into adipose biology.
Key publications:
Mapping the in vivo adipogenic trajectory and the underlying regulatory networks at single-cell resolution
Lukas Mc.Kenzie Oussoren*, Babukrishna Maniyadath*, Uthpala M L, Alexander Hinz, Ellen Gammelmark Klinggaard, Isabell Victoria Strandby Ernst, Elvira Laila Van Hauwaert, Rasmus Rydbirk, Jesper Grud Skat Madsen, Susanne Mandrup
Plasticity of White Adipose Tissue Depots at Single-Cell Level during Obesity Regression in Mice
Ellen Gammelmark Klinggaard, Babukrishna Maniyadath, Flora Reincke Fuglsang, Lukas Mc.Kenzie Oussoren, Raquel Teixeira Saborano, Felix Boel, Vyacheslav Akimov, Kenneth Lewis, Daniel Hansen, Kim Ravnskjær, Ormond MacDougald, Blagoy Blagoev, Søren Fisker Schmidt, Susanne Mandrup
Lipolysis is Required for Differentiation and Maintenance of the Adipocyte
Lasse Kruse Markussen*, Linda Gimm*, Lukas Mc.Kenzie Oussoren, Renate Schreiber, Sif Sofie Dahl, Niels Jakob Larsen, Oliver Bonde von Zwol, Ann-Britt Marcher, Jacob Bo Hansen, James Granneman, Rudolf Zechner, Susanne Mandrup
Adipose tissue cell states reflect progression and clinical subtypes of MASLD in human obesity
Johanne Drewsen Madsen*, Trine Vestergaard Dam*, Jyotsna Nambiar, Chunyu Liang, Aino Rakel Peltonen, Rasmus Rydbirk, Lukas Mc.Kenzie Oussoren, Kamilla Schlippe Jakobsen, Ellen Gammelmark Klinggaard, Daniel Hansen, Babukrishna Maniyadath, Susanne Mandrup, Anne Loft, Søren Fisker Schmidt.
Contact Information
- Email: lukasmo@bmb.sdu.dk
- LinkedIn: https://www.linkedin.com/in/lukas-oussoren-4bb941243/