Digestion in our intestines includes bile salts, buffer substances, and enzymes. Human aspirates are compared with artificial media that shall mimic human fluids. We study the interaction between drugs and these media, and their influence on drug absorption.
For this purpose, the colloidal particles that are developed, are separated and quantified by a chromatographic method (asymmetric field-flow fractionation, A4F).
Elvang, P.A., Bohsen, M.S., Stein, P.C., Bauer-Brandl, A., Riethorst, D., Brouwers, J., Augustijns, P., Brandl, M.: Co-existing colloidal phases of human duodenal aspirates: Intraindividual fluctuations and interindividual variability in relation to molecular composition (2019) Journal of Pharmaceutical and Biomedical Analysis, 170, pp. 22-29.
Bibi, H.A., Holm, R., Bauer-Brandl, A. Simultaneous lipolysis/permeation in vitro model, for the estimation of bioavailability of lipid based drug delivery systems. (2017) Eur J Pharm Biopharm, 117, pp. 300-307. DOI: 10.1016/j.ejpb.2017.05.001
Elvang, P.A., Stein, P., Bauer-Brandl, A., Brandl, M: Characterization of co-existing colloidal structures in fasted state simulated intestinal fluids FaSSIF: A comparative study using AF4/MALLS, DLS and DOSY. (2017) J Pharm Biomed Anal, 145, pp. 531 – 546. https://dx.doi.org/10.1016/j.jpba.2017.07.014
Elvang, P.A., Hinna, A.H., Brouwers, J., Hens, B., Augustijns, P., Brandl, M. Bile Salt Micelles and Phospholipid Vesicles Present in Simulated and Human Intestinal Fluids: Structural Analysis by Flow Field–Flow Fractionation/Multiangle Laser Light Scattering. (2016) Journal of Pharmaceutical Sciences, 105 (9), pp. 2832-2839. DOI: 10.1016/j.xphs.2016.03.005