Our Expertise
- Whole blood
- Whole blood monocytes
- Bone marrow
- Bone marrow monocytes
- Out-growth cultures from fresh human trabecular bone specimens
- Fresh human bone-marrow derived mesenchymal stem cells
- Standard conditions with FBS, M-CSF and RANKL
- Restricted conditions only with M-CSF and without FBS and RANKL
- Human osteoclast mono-culture
- Human osteoclast/osteoblast co-culture
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For more examples on our time-lapse recording of bone resorption please refer to:
- Time-lapse reveals that osteoclasts can move across the bone surface while resorbing | Journal of Cell Science | The Company of Biologists
- Catabolic activity of osteoblast lineage cells contributes to osteoclastic bone resorption in vitro | Journal of Cell Science | The Company of Biologists
- IJMS | Free Full-Text | Osteoclasts’ Ability to Generate Trenches Rather Than Pits Depends on High Levels of Active Cathepsin K and Efficient Clearance of Resorption Products (mdpi.com)
- HBP/O-GlcNAcylation Metabolic Axis Regulates Bone Resorption Outcome - T.M. Taira, E.S. Ramos-Junior, P.H. Melo, C.C. Costa-Silva, M.G. Alteen, D.J. Vocadlo, W.B. Dias, F.Q. Cunha, J.C. Alves-Filho, K. Søe, S.Y. Fukada, 2023 (sagepub.com)
- GIP reduces osteoclast activity and improves osteoblast survival in primary human bone cells | European Journal of Endocrinology | Oxford Academic (oup.com)
- For examples on our time-lapse recording of osteoclast fusion and how we analyze them, please refer to:
Original publication can be found at: Septins are critical regulators of osteoclastic bone resorption
Original publication can be found at: Catabolic activity of osteoblast lineage cells contributes to osteoclastic bone resorption in vitro | Journal of Cell Science | The Company of Biologists