Introduction:
Inverted spinning-disk confocal system for high frame rate confocal imaging in light-sensitive or live-cell applications. The microscope setup encompasses environmental control with temperature, humidity and CO₂ control.
Main features and applications:
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Live-cell imaging with stable temperature, humidity and CO₂ control.
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High-speed spinning disk confocal imaging across multiple excitation lines.
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Piezo Z-stage enables fast/accurate imaging in the z-dimension.
Specifications:
Microscope body:
Nikon Ti-E inverted microscope with Perfect Focus System (PFS).
Compatible sample holders:
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Standard ~25 x 75 mm glass slides + chambered glass slides
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Culture dishes w. diameter: ~30 – 40 mm
Scan mechanism:
Yokogawa CSU-X1 spinning disk; 50 µm pinholes (optimal for 60×/100×).
Light Sources:
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Lumencor Celesta Quattro 4ch with Despeckler, lines 405 nm, 476 nm, 555 nm and 637 nm with ~800 mW output per color channel (installed September 2025).
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Nikon Intensilight for standard EPI illumination when using the eyepiece.
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Diascopic transmission illumination
Objectives:
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20× / NA 0.5
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40× / NA 0.75
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60× / NA 1.4 oil
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100× / NA 1.4 oil
Camera:
Andor iXon EMCCD DU-885 (1004 × 1002 px, 8 × 8 µm) (may be replaced with an Andor Zyla sCMOS).
Stage & Environment:
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Motorized stage and objective for automated multi-point imaging
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Nikon Perfect Focus System (PFS) for hardware-based autofocus
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Prior piezo Z-stage for fast/precise imaging in Z
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OkoLab cage incubator for controlling humidity, temperature and CO₂ levels.
Software:
NIS-Elements (acquisition) with ND acquisition (and JOBs??).
Location:
V10-602a-0