Features
- >Stochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution image by combining precise localization information for individual fluorophores in microscope specimens. This multi-color localization data in three dimensions (xyz) generates super-resolved images at 10 x the resolution of conventional light microscopes (up to 20 nm in x,y, 50 nm in z). This enables visualization of molecular interactions and organizations on the nanoscale.
- Acquisition times down to the ~10 seconds-range per frame is possible (high-speed mode, 20 µm x 20 µm area).
- Camera ORCA-Flash 4.0 sCMOS camera (Hamamatsu). Field of view is up to 80 µm x 80 µm.
- The LU-NV laser unit contains the following lasers: 405 nm, 488 nm, 561 nm, 647 nm.
- The Nikon N-SIM is build onto a Ti-2 LFOV microscope body available with the following objectives: 100x Oil, 100x Si.
- NB. operating conditions are restricted to 20-25°C (+/- 0.5°C) i.e. temperature fluctuations are not tolerated well.
- The microscope PC is installed with Nikon NIS-Elements that is also used on other DaMBIC microscopes allowing easy familiarization with the user environment across the different microscopes.
- Overview of the Nikon super resolution microscopes (SIM and STORM).
>Location: V10-602a-0
