Nikon AX R MP Multiphoton Confocal Microscope (Inverted)
Introduction
Our inverted Nikon AX R MP is a high-performance multiphoton/confocal platform designed for deep, low-photodamage imaging in living and fixed samples. Optimized for multiphoton imaging (2-photon and 3-photon) and SHG, with a tunable laser (660–1320 nm) and fast resonant scanning, it spans macro-to-micro imaging; ideal for live-cell dynamics, tissue slices, organoids, and intravital-style preparations on an inverted stand.
Application examples
• Intravital deep imaging of brain and tumors, immune cell tracking.
• Thick tissues and slices including brain, dermis, and tumor/xenograft sections.
• Imaging of Organoids and 3D cultures.
• Deep lipid contrast with 3-photon for myelinated axons, adipose tissue, and lipid droplets
• Label-free structure with SHG for collagen and myosin in skin, tendon, cornea, and stroma
• Fast dynamics such as calcium signals, blood flow, and leukocyte rolling via resonant scanning
• Multicolor separation using spectral unmixing to handle autofluorescence and crosstalk
Imaging Modalities
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Two-photon & three-photon excitation for deep tissue imaging with reduced photobleaching/phototoxicity.
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Second Harmonic Generation (SHG) for label-free structure (e.g., collagen, myosin).
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Confocal imaging with visible lasers for high-resolution surface and subsurface structures.
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High-speed dynamics: resonant scanning up to 720 fps (ROI), with a large FOV for fast tiling and time-lapse.
Specifications
Microscope body & stage
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Nikon ECLIPSE Ti2-E inverted platform with motorized encoded Z and Perfect Focus System (PFS).
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Motorized XY stage with joystick and encoded positioning; supports slides, dishes, chambered coverglass, and multiwell plates.
Objectives (selected)
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4× Plan Achromat (NA 0.10, WD 30 mm)
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10× Plan Apo Lambda D (NA 0.45, WD 4.0 mm)
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20× Apochromat LWD Lambda S WI (NA 0.95, WD 0.95 mm)
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40× Apochromat LWD Lambda S WI (NA 1.15, WD 0.60 mm)
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60× Plan Apo Oil (NA 1.42, WD 0.15 mm)
Lasers & excitation
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Multiphoton IR: tunable 660–1320 nm with an additional fixed 1040 nm line; phase-locked beams support advanced multiphoton/label-free modalities.
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Visible (confocal) lasers: 405, 488, 561, 640 nm.
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Widefield LED: 4-channel LED (centers ~385, 488, 550, 621 nm) for brightfield/epi-fluorescence.
Scanners
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Resonant: up to 720 fps (ROI), 2K × 2K imaging.
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Galvano: high-resolution imaging up to 8192 × 8192 pixels.
Detectors
Non-descanned detectors (NDD, multiphoton)
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4-channel NDD configuration including GaAsP PMTs for high sensitivity.
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Filters in front of the NDD (installed cubes): 450/70, 550/88, 641/75, 732/68 — optimized for typical multiphoton emission bands and SHG.
DUX-VB4 (visible/confocal & IR-compatible spectral detection)
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4-channel tunable detector using an LVF (Linear Variable Filter) for continuously adjustable detection windows.
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Spectral control per channel: ~5 nm wavelength resolution, 1 nm step setting, 10–320 nm adjustable bandwidths—ideal for spectral separation/unmixing.
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PMT configuration: 2 × GaAsP and 2 × multi-alkali; the two GaAsP channels are configured for spectral detection to maximize sensitivity for weak signals.
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Fixed emission filters installed (for quick access):
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PMT 1: 452/45 nm (DAPI band)
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PMT 4: 700/75 nm (Cy5 band)
Software
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NIS-Elements with ND acquisition and JOBS workflows (multipoint, tiling, Z/T, multi-wavelength), Denoise.ai, and spectral unmixing; supports FRAP/FLIP/photoactivation and advanced 3D/4D analysis.