Leica SP8 microscope combines possilibty to perform:
- Stimulated emission depletion microscopy (STED)
- Coherent anti-Stokes Raman spectroscopy (CARS)
- Fluorescent confocal microscopy.
Special features
- Flexible excitation (470-700 nm)
- Filter-free spectral detection
- Simultaneous 3-colour detection,
- Super-resolution STED at 592 nm (green dyes),
- Resonant scanners
- Gated detection.
- NDD detectors
Confocal
The system is a standard laser scanning confocal microscope. Excitation: The system is equipped with a white light laser (WLL) for a flexible choice of up to eight simultaneous excitation wavelengths (470-700 nm). The system further has a choice for argon laser excitation at 458 nm, 476 nm, 488 nm, 496 nm, and 514 nm.
Detection
Emission wavelength collection is flexible because of the filter-free spectral detection system. The system is equipped with three point detectors; two PMT detectors and one hybrid (HyD) detector. The HyD detector has gated detection.
Objectives
HC PL IRAPO 40x/1.10 WATER, HC PL APO CS2 40x/1.10 WATER, HC PL APO CS2 63x/1.40 OIL, HCX PLAPO 100x/1.40 OIL, HC PL APO CS2 20x/0.75 DRY, HC PL APO CS2 10x/0.40 DRY.
Further features
The system features eg. multiple ROI selection, scan speeds up to 7 frames/s (512x512 pixels), resonant scanner (8 kHz) with scan speeds of up to 28 frames/s (512 x 512 pixels), xyz, xzy, xyzt, xzyt, and lambda scan modes (both excitation and emission).
STED
The STED (STimulated Emission Depletion) systems is used with the above confocal system. Depletion: The system is equipped with a 592 nm continuous wave (CW) STED laser allowing for super-resolution microscopy. Sub-diffraction limited spatial (xy) resolution of down to ~50 nm standard green emitting STED dyes (eg. alexa488, Atto-488, abberior star 488, chromeo488).
Objective
100X/1.4.
CARS and Multiphoton
laser 1064nm,
Compatible objectives: 20x Air (IMM) or 40x IRAPO water.
Manual for microscope usage Leica STED-CARS-manual.pdf
Software for data analysis can be found here Leica LAS X
Location: V12-604-0