STimulated Emission Depletion (STED)
Introduction:
STED (STimulated Emission Depletion) nanoscopy module providing super-resolution imaging with far-red dyes. Typical resolutions are below 30 nm (down to ~20 nm), with options for 2-color STED, STED-FLIM and Matrix detection.
Applications:
- Super-resolution fluorescence imaging with fluorescent proteins (e.g., mCherry) and organic dyes (e.g., abberior STAR Red/580/635P).
- Two-color STED for multichannel nanoscale imaging.
- FLIM alone- or in combination with STED for lifetime-based contrast.
- Matrix detection for background reduction and improved optical sectioning
Specifications
Microscope body:
Olympus IX83 inverted microscope with 4-color LED illumination.
Compatible sample holders
Standard ~25 x 75 mm glass slides + chambered glass slides
Culture dishes w. diameter: ~30 – 40 mm
Objectives:
60x / NA 1.42 oil objective suitable for STED imaging
Light Sources:
- Pulsed (25 ns) STED depletion laser at 775 nm (suitable for depleting e.g. Abberior STAR Red and STAR Orange).
- Pulsed excitation lasers: 561 nm and 640 nm
- Mai-Tai pulsed (12.5 ns) laser tunable from 780 to 920 nm for multiphoton excitation (nb. start-up should be in the 80 MHz configuration). 4-color LED for epifluorescence/brightfield
Detectors:
- High quantum efficient APDs for detecting low count rates and for single photon counting
- Matrix detection unit with over 20 single detection elements each capable of single photon counting
Software:
- Abberior LiGHTBOX is a user-friendly, routine-use interface for microscope operations and with a small set of post-processing tools
- iMSPECTOR offers advanced user the flexibility for control of lower-level machine settings, for complex tasks and experimental design.
Location: V12-603a-0