Abberior MINFLUX

MINimal flourescence photon FLUXes nanoscopy (MINFLUX)

Traditional fluorescence microscopy is limited by the diffraction limit of light, which restricts resolution to about 200-300 nanometers. MINFLUX breaks this limit and allows imaging at much higher resolutions (down to a few nanometers).

MINFLUX

• Tracks labeled molecules at high frequencies up to 10 kHz, resolving molecular motion every 100 μs
• Achieves localization precisions of 1-3 nm (3D) in biological samples on large fields of view (10 x 15 μm)
• Compatible with both live and fixed fluorescently labeled specimens. Multi-color imaging is possible.

Comparison between confocal and MINFLUX resolution. Individual RNA probes hybridized along an RNA strand. © 2024 DaMBIC.

MINFLUX principle

MINFLUX works by the principle of minimal photon fluxes where fluorophore positions can be determined with a minimal number of photons and consequently within a fast and accurate spatio-temporal regime. The single emitting molecules are localized with an excitation pattern featuring a spatially well-controlled intensity zero, e.g. a donut as seen below. The intensity zero of this excitation beam is then scanned onto the sample and used to probe the fluorophore positions.

System details

• Resolution down to 1-3 nm
• The system contains two pulsed excitation laser sources @ 561 nm and @ 640 nm allowing for imaging of fluorophores in the excitation range ~550 - 680 nm.
• The MINFLUX is built onto an IX83 Olympus microscope with a multicolor CoolLED illumination source.

Specifications

Microscope body:

At DaMBIC, the MINFLUX is built on an Olympus IX83 inverted microscope with a multicolor CoolLED illumination source.

Sample holder (compatible with):

High-NA objective set optimized for MINFLUX.

Light Sources:

Integrated single-photon-sensitive detectors for MINFLUX localization.

Stage:

Imspector (acquisition and control).

Location:

V12-605b-0