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Seeing is believing, which is why microscopy and imaging play traditionally a central role in biological research. At the Bioimaging section we apply state of the art imaging techniques and analysis to obtain quantitate spatiotemporal information on a variety of specimens. Problems range from investigation of protein and cholesterol transport in health and disease cells over cancer diagnostics and treatment to drug delivery and characterization of biomaterials such as spider silk or 3D printed tissue. Particular focus is on sensitive optical imaging of processes taking place in living cells in their native context. Using latest fluorescence technology, we are able to follow biochemical processes in living cells at the level of single molecules. Insights obtained by such approaches is currently revolutionizing central concepts in molecular cell biology.