The Flow Cytometers
Becton Dickinson LSR II Flow Cytometer:
It is equipped with four lasers:
Violet 405nm, blue 488nm, yellow-green 561nm and a red 639nm.
- The 405nm and 639nm laser are capable of collecting up to two colors.
- The 561-nm octagon array is capable of collecting up to four colors.
- The 488nm laser is capable of collecting up to two colors and side scatter
The configuration with fluorochromes are variable, but the equipment is at present:
- 405nm laser: AmCyan, Qdot 525, DAPI, Hoechst, V450, BrilliantViolet421, Pacific Blue…
- 488nm laser: FITC, Alexa Flour 488, GFP, CFSE, PerCP, PERCP-CY5,5, PE-CY7 …
- 639nm laser: APC, Alexa Fluor 647, APC-Cy7, APC-H7…
- 561nm laser: PE, DsRed, PI, PE-Texas Red, PE Alexa Fluor 610, HcRed, PE-Cy7, PE-Cy5,5
LSR II is also equipped with HTS (High Throughput Sampler) and able to sample from a 96- or 384-microtiter tray.
The Cell Sorter
Becton Dickinson FACSAria III Cell Sorter and Analyser:
It is equipped with four lasers:
Violet 405nm, blue 488nm, yellow-green 561nm and a red 639nm.
- The 405nm octagon can detect up to six colors
- The 488nm laser can collect two colors and side scatter
- The 561nm octagon array is capable of colleting up to five colors
- The 633nm laser cant collect up to three colors.
The configuration with fluorochromes are variable, but the equipment is at present:
- 561nm laser: PE-Alexa Fluor 700, PE-Cy5-5, PE-Texas Red, PI, mCherry, PE-Cy7, PE-Cy5, PE
- 405nm laser: QDot705, Qdot 605, DAPI, Indo-1, Pacific Blue, QDot 800, Qdot 655, AmCyan
- 488nm laser: PerCP-Cy5-5, FITC, Alexa fluor 488
- 633nm laser: APC-Cy7, APC-H7, APC, Alexa Fluor 700
The Cytometer is equipped for two-way and four-way sorting, sorting of cells into 6-well, 24 well or 96-well plates, as well as for single cell cloning. Sorting rates of up to 70,000 cells per second are achievable.
Software and Data Analysis Workstations:
- The LSR II and the FACSVantage/Diva cell-sorter employ PC-based DIVA software.
- Data analysis workstations (PC and MAC), accessing FACSCORE shared Network server, are available for data processing with Cell Quest Pro software, DIVA-software and FLOWJo-software.