Equipment

 Optical Imaging Facility:  

The Optical Imaging part of the Core Facility includes two high performance Confocal Laser Scanning Microscopes, one Laser Capture Microdissection system and one in-vivo optical imaging instrument, IVIS.

Microscope location: J. B. Winsløws Vej 21 ground floor East, room 0.56.

IVIS location: J. B. Winsløws Vej 23, room 0.34.  

 

Olympus FV1000MPE, Upright Single- and Multiphoton Fluorescence Microscope

 

Excitation lasers: 405, 458, 488, 515, 559, 635, and a MaiTai DeepSee Titanium-sapphire laser (690-1040nm), which allows for imaging of structures at depths up to 1.1mm.  

The Microscope includes the following options:  
Multiphoton excitation allows for investigations on thick living tissue specimens that would not otherwise be possible with conventional imaging techniques.

Simultaneous Laser Light Stimulation and Imaging for a variety of applications, including FRAP, FLIP, photo-activation, photo-conversion, uncaging, laser ablation and many others.    

Differential Interference Contrast revealing the topographical profile of the specimen.    

 

Zeiss LSM 710, Inverted Singlephoton Fluorescence Microscope with CO2, Temp and O2 module    

 

Excitation lasers: 405, 458, 488, 514, 543, 633.  

The Microscope includes the following options:  
The CO2, Temp and O2 module is optional and provides stabilization of the “in vitro environment” for cells and tissue culture with the option of live cell imaging using time-lapse option.  

Differential Interference Contrast revealing the topographical profile of the specimen.  

 

 IVIS Spectrum, pre-clinical Optical Imaging (in vivo imaging system)  

 

The IVIS uses an optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell-trafficking and gene-expression patterns in live animals. You have the advantage of bioluminescent and fluorescent reports across the blue to near infrared wavelength region. Also single-view 3D-tomography is provided.

 

Laser Capture Microdissection (LCM), Olympus MMI CellCut + Arcturus/Olympus PixCell Lle

LCM is typically used to isolate ROIs (regions of interest) from tissue or single cells for further cloning, re-cultivation, manipulation or downstream analysis.

We deeply appreciate for Trevor Owens and Henrik Ditzel hereby providing  us with these instruments.

 

Software and Data Analysis Workstations:

The FV1000MPE employ PC-based FV-ASW Olympus software.  It also employs a Bitplane IMARIS software.

The Zeiss LSM710 employ ZEN Zeiss software.

Data analysis workstation (PC), accessing shared Network server, are available for data processing.

 

 

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