Menu

Equipment

 

The Flow Cytometry Facility is equipped with two analytical Flow Cytometers and a high-speed Cell Sorter:

 

Becton Dickinson LSR II Flow Cytometer

Equipped with four lasers:

Violet 405nm, blue 488nm, yellow-green 561nm and a red 639nm.

The 561-nm octagon array is capable of collecting up to four colors. The 488nm laser two colors and side scatter, the 405nm and 639nm laser two colors.

The configuration with fluorochromes are variable, but the equipment is at present:

  • 405nm laser: AmCyan, Qdot 525, DAPI, Hoechst, V450, BrilliantViolet421, Pacific Blue…
  • 488nm laser:  FITC, Alexa  Flour 488, GFP, CFSE, PerCP, PERCP-CY5,5, PE-CY7 …
  • 639nm laser: APC, Alexa Fluor 647, APC-Cy7, APC-H7…
  • 561nm laser: PE, DsRed, PI, PE-Texas Red, PE Alexa Fluor 610, HcRed, PE-Cy7, PE-Cy5,5

LSR II is also equipped with HTS (High Throughput Sampler) and able to sample from a 96- or 384-microtiter tray.

 

The Cell Sorter:

Becton Dickinson FACSAria III Cell Sorter and Analyser

 

 

Equipped with four lasers:

Violet 405nm, blue 488nm, yellow-green 561nm and a red 639nm.

The 561nm octagon array is capable of colleting up to five colors. The 405nm octagon can detect up to six colors, the 488nm laser two colors and side scatter and the 633nm laser up to three colors.

The configuration with fluorochromes are variable, but the equipment is at present:

  • 561nm laser: PE-Alexa Fluor 700, PE-Cy5-5, PE-Texas Red, PI, mCherry, PE-Cy7, PE-Cy5, PE
  • 405nm laser: QDot705, Qdot 605, DAPI, Indo-1, Pacific Blue, QDot 800, Qdot 655, AmCyan
  • 488nm laser: PerCP-Cy5-5, FITC, Alexa fluor 488
  • 633nm laser: APC-Cy7, APC-H7, APC, Alexa Fluor 700

The Cytometer is equipped for two-way and four-way sorting, sorting of cells into 6-well, 24 well or 96-well plates, as well as for single cell cloning. Sorting rates of up to 70,000 cells per second are achievable.

  

Software and Data Analysis Workstations:

The LSR II and the FACSVantage/Diva cell-sorter employ PC-based DIVA software.

Data analysis workstations (PC and MAC), accessing FACSCORE shared Network server, are available for data processing with Cell Quest Pro software, DIVA-software and FLOWJo-software.

To give you the best possible experience, this site uses cookies Read more about cookies

Accept cookies