Applied methodologies, techniques and facilities

  • Laser capture microdissection
  • Real-time PCR
  • Magnetic bead cell sorting
  • Primary culture of mouse neurons and glial cells
  • In vitro assays for T cell activation
  • ELISA
  • Experimental autoimmune encephalomyelitis
  • Cuprizone demyelination
  • Stereotactic axonal lesion in mouse brain
  • Facial nerve lesion in mouse
  • Flow cytometric sorting of leukocytes and glial cells from mouse central nervous system
  • Replication-defective adeno- and lentivirus purification and titering
  • Adeno- and lentiviral vector gene delivery to the mouse central nervous system

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