Pernille B. Lærkegaard Hansen
Phone: +45 65503717
- Role of calcium channels for blood pressure regulation and kidney function studied in human tissue and transgenic mice.
- Aldosterone, mast cells and endothelial function in mouse and human.
The main interests of the lab are the vasculature of the kidney, kidney function and blood pressure regulation. Renal arteriolar function influence regulation of renal blood flow, glomerular filtration rate and salt and water homeostasis and thereby blood pressure. Furthermore, the kidney vascular segments are involved in several pathological conditions such as diabetes and hypertension.
Calcium channel blockers are widely used for treatment of hypertension as they inhibit voltage-gated calcium channels (Cav). The family of Cav consists of several subfamilies and we have previously elucidated the involvement of Cav in contraction of renal blood vessels. We investigate the importance of these channels in kidney function, blood vessels function and blood pressure regulation in man and mouse.
Aldosterone affects blood vessels under normal conditions but are also involved in the pathogenesis during hypertension and other vascular diseases. We have investigated the effect of aldosterone on renal blood vessels and we have discovered a new mechanism for the contractile response to aldosterone involving histamine release. We plan to study this new phenomenon and the mechanisms involved in aldosterone induced histamine release.
Evidence from our animal research form basis for our translational research using human material and our projects will provide new insights to human kidney function.
Methods and Techniques
- Blood pressure and kidney function determined in conscious mice
- Perfused resistance blood vessels
- Small vessel pressure myography
- Microdissection of nephron segments and renal microvessels
- Cell culture
- Confocal microscopy/Ca2+-imaging
- Human kidney tissue studies
- Pharmacological intervention with drug delivery in mice
- Transgenic and knock-out mice
- Metabolic cage studies of mice
- Anne Thuesen,
- Kristina Lyngsø,
- Louise Rasmussen,
- Rasmus terp,
- Kasper Assersen,
- Vivi Monrad.
- Physiology for medical, biomedical and pharmacy students.
- The Danish Research Council for Health and Disease
- The Lundbeck Foundation
- The Danish Heart Research Association
- The AP Moller Foundation
- The Aase and Ejnar Danielsens Fond
- The Danish Kidney Association
- Caryl Hill, The Australian National University, Australia.
- Frederic Jassier, Cordeliers Research Centre, France.
- Jean Chemin, Institut de Génomique Fonctionnelle, France.
- Paul Vanhoutte, University of Hong Kong, China.
- Lars J. Jensen, Copenhagen University.
- Collaborations with Odense University Hospital; Center for Individualized Medicine in Arterial Diseases (Lars Melholt Rasmussen), Department. of Nephrology (Claus Bistrup), Urology (Anja Toft) and Patology (Niels Marcussen).
Membership in Societies
- Danish representative in the Management Committee of the EU network COST ADMIRE
- Board member of the Scandinavian Physiological Society
- American Physiological Society
- The Scandinavian Physiological Society
- Danish Cardiovascular Research Academy
- Danish Pharmacological Society
- Danish Hypertension Society
36. Poulsen CB, Al-Mashhadi RH, Cribbs L, Skøtt O, Hansen PB. T-type voltage gated calcium channels are involved in vasodilation and vasoconstriction in mouse efferent arteriole. Kidney Int. 2011,79(4):443-51
37. Andersen H, Jaff MG, Høgh D, Vanhoutte P, Hansen PB. Adenosine elicits an eNOS-independent reduction in arterial blood pressure in conscious mice that involves adenosine A(2A) receptors. Acta Physiol (Oxf). 2011 Sep;203(1):197-207
39. Hansen PB, Poulsen CB, Walter S, Marcussen N, Cribbs LL, Skøtt O, Jensen BL. Functional importance of L- and p/q-type voltage-gated calcium channels in human renal vasculature. Hypertension. 2011 Sep;58(3):464-70.
46. Hansen PB. Functional and pharmacological consequences of the distribution of voltage-gated calcium channels in the renal blood vessels. Acta Physiol (Oxf). 2013 Apr;207(4):690-9.
48. Schjerning J, Uhrenholt TR, Svenningsen P, Vanhoutte PM, Skott O, Jensen BL, Hansen PB. Histamine-dependent prolongation by aldosterone of vasoconstriction in isolated small mesenteric arteries of the mouse. Am J Physiol Heart Circ Physiol. 2013 Apr 15;304(8):H1094-102
50. Howitt L, Kuo IY, Ellis A, Chaston DJ, Shin HS, Hansen PB, Hill CE. Chronic deficit in nitric oxide elicits oxidative stress and augments T-type calcium-channel contribution to vascular tone of rodent arteries and arterioles. Cardiovasc Res. 2013 Jun 1;98(3):449-57
55. Cazade M, Bidaud I, Hansen PB, Lory P, Chemin J. 5,6-EET potently inhibits T-type calcium channels: implication in the regulation of the vascular tone. Pflugers Arch. 2013 Dec 11. [Epub ahead of print]
56. Ivana Y, Kuo, Howitt L, Sandow S, McFarlane A, Hansen PB, Hill, CE. Role of T-type channels in vasomotor function: Team player or chameleon? Pflügers Arch 2014;466(4):767-79.
57. Svenningsen P, Andersen K, Thuesen A, Shin HS, Vanhoutte PM, Skott O, Jensen BL, Hill C, Hansen PB. T-type Ca2+ Channels Facilitate NO-formation, Vasodilatation and NO-mediated Modulation of Blood Pressure. Pflügers Arch 2014 In press
58. Eskildsen MP, Hansen PB, Stubbe J, Toft A, Walter S, Marcussen N, Rasmussen LM, Vanhoutte PM, Jensen BL. Prostaglandin I2 and Prostaglandin E2 Modulate Human Intrarenal Artery Contractility Through Prostaglandin E2-EP4, Prostacyclin-IP, and Thromboxane A2-TP Receptors. Hypertension. 2014 Jun 9 [Epub ahead of print]
59. Thuesen, A, Andersen, H, Cardel M, Toft, A, Walter S, Marcussen N, Jensen BL, Bie P, Hansen PB. Differential effect of T-type voltage-gated calcium channel disruption on renal plasma flow and glomerular filtration rate in vivo. AJP renal, in press 2014