The research conducted in the group of Henrik Dimke can be divided into two lines. Focus is directed towards 1) elucidating the regulatory mechanisms involved in maintaining mineral balance (Calcium, Magnesium, and Phosphate) via trans- and paracellular pathways in kidney and intestine. In particular, regulation of poreforming claudins as well as epithelial channels and transporters involved in mineral transport is of interest. 2) Moreover, additional studies are focusing on understanding the role of the endothelium in renal physiology and various forms of kidney disease.
Methods and Techniques
Primary experimental approaches used to study the regulation of these molecular mechanisms, include the use of various targeted transgenic models, histological and molecular biological techniques, in addition to human kidney isolates. These are outlined below.
- Immunohistochemistry (light-, laser scanning confocal-, multi-photon microscopy)
- Cell culture (Transient and stable expression systems)
- Western blotting
- Quantitative PCR
- Studies on human kidney tissue
- Various transgenic and cell-specific knockout models
- Metabolic studies of renal function in animals
- Developmental changes in renal morphology and function.
- Antibody development and characterization
About 1 Master student and 1-2 Bachelor students per year. Current group members include a Research Technician and post doctoral researcher.
Bachelor module 7 for Medical students, Reproduction and pharmacodynamics
Renal Physiology for nephrologists in-training – Sundhedsstyrelsen
Associate Professor Sebastian Frische (University of Aarhus, DK)
Associate Professor Todd Alexander (University of Alberta, Canada)
Associate professor Karsten Skjødt (SDU)
Senior Researcher Hans Christian Beck (SDU)
Professor Susan Quaggin (Northwestern University, Feinberg School of Medicine, Chicago, USA)
Membership in Societies
American Physiological Society
American Society of Nephrology
The American Heart Association's Council on the Kidney in Cardiovascular Disease
Scandinavian Physiological Society/Nordisk Fysiologisk Förening
Danish Cardiovascular Research Academy (DaCRA)
Member of the Young Academy of the Royal Danish Academy of Sciences and Letters.
Alexander RT, Beggs MR, Zamani R, Marcussen N, Frische S, Dimke H. 2015. Ultrastructural and Immunohistochemical Localization of the Plasma Membrane Ca2+-ATPase 4 (PMCA4) in Ca2+-Transporting Epithelia. Am J Physiol Renal Physiol. In Press, IF(2014)=3.248.
Dimke H, Sparks MA, Thomson BR, Frische S, Coffman TM, Quaggin SE. 2015. Tubulo- Vascular Crosstalk by Vegfa Maintains Peritubular Microvasculature in Kidney. J. Am. Soc. Neph. 26:1027-38. IF (2014)=9.343.
Alexander RT, Rievaj J, Dimke H. 2014. Paracellular Calcium Transport Across Renal and Intestinal Epithelia. Biochemistry and Cell Biology. 92:467-80. IF= 2.915.
Dimke H, Desai P, Borovac J, Lau A, Pan W, Alexander RT. 2013. Activation of the Ca2+-Sensing Receptor Increases Renal Claudin-14 Expression and Urinary Ca2+ Excretion. Am J Physiol Renal Physiol. 304:F761-769. IF=3.300.
Dimke H*, San-Cristobal P*, de Graaf M, Lenders JW, Deinum J, Hoenderop JG, Bindels RJ. 2011. γ-Adducin functions as a novel regulator of the thiazide-sensitive NaCl-cotransporter. J. Am. Soc. Neph. 22:508-17. *Authors contributed equally. IF=9.663
Dimke H, vd Wijst J, Alexander TR, Meijer IMJ, Mulder GM, van Goor H, Tejpar S, Hoenderop JG, Bindels RJ. 2010. Effects of the EGFR Inhibitor Erlotinib on Magnesium Handling. J. Am. Soc. Neph. 21: 1309-1316. IF= 8.288