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Applied methodologies, techniques and facilities

Gene expression array
Quantitative real-time PCR and low density arrays (LDA)
MicroRNA profiling

Fractionation of cell content using gradient centrifugation
Membrane protein purification
Protein purification from tissue
Protein digest and stage tip purification of samples for mass spectrometry

Antibody Phage display technology for generation of human monoclonal antibodies
Production, purification and characterization of mouse monoclonal antibodies
Antibody conjugation

Protein characterization
SDS-PAGE and immunoblotting
FACS analysis

Cell culturing
Mammosphere formation assay
Cell sorting using flow cytometry
siRNA-mediated knock-down

General molecular biological techniques: Cloning, PCR, Sequencing
Expression of recombinant proteins in mammalian, yeast and bacterial expression systems

Laser capture microdissection of tissues
Tissue microarrays
Gene characterization

Last Updated 16.08.2016