Training and Expertise

 The core facility staff is experienced in sorting on the basis of

  • phenotypic markers
  • cell cycle
  • DNA-binding dye exclusion (“side-populations”)
  • calcium ion flux 

as 2-way, 4-way or clonal (96-well) sorts.

In terms of analytical Flow Cytometry, the unit is well versed in

  • cell phenotyping
  • marker quantitation
  • cell proliferation analyses
  • intracellular probing
  • cell cycle analysis
  • measure of cytokines with CBA or FlowCytoMix
  • measurement of apoptosis

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