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Experimental facilities

SDU currently host several imaging centers, among them are:

Microscopes list (not complete yet):

Electron  and Ion microscopes:

  1. SEM (Hitachi S-4800)
  2. SEM (Fei Quanta 200)+EDX E(nergy-dispersive X-ray spectroscopy)
  3. TEM (Transmision Electron Microscope, JEOL 1400 plus)
  4. Zeiss ORION NanoFab Helium Ion Microscope

Light microscopes:

  1. Zeiss LSM510
  2. Leica SP8 for Stimulated emission depletion microscopy (STED), Coherent anti-Stokes Raman spectroscopy (CARS) and fluorescent confocal microscopy
  3.  Spinning disk microscope
  4. Olympus TIRF microscope: total internal reflection
  5. Olympus multiphoton microscope
  6. Nikon Eclipse Ti-E inverted Widefield microscope
  7. Nikon two photon FLIM (fluorescence life time microscopy)
  8.  Nikon 2 photon home built STED (Stimulated emission depletion microscopy )
  9. Zeiss LSM 710
  10. Olympus FV1000MPE
  11.  Zeiss Axio Imager M1 Microscope
  12.  IVIS Spectrum, pre-clinical Optical Imaging (in vivo imaging system)
  13. Laser Capture Microdissection (LCM), Olympus MMI CellCut + Arcturus/Olympus PixCell Lle

Atomic force microscopes:

  1. Veeco Dimension 3100,
  2. JPK NanoWizard at NanoSYD and at FKF

CT scanners:

  1. PET CT (Positron Emission Tomography, Computed Tomography) system combines the Inveon™ Docked PET scanner with an Inveon SPECT•CT system.
  2. vivaCT 40 - Small animal in vivo preclinical micro-CT scanner
  3. Semi-nano-CT sample scanner

Another equipment:

  1. MALDI TOF MS instrument 
  2. Perkin Elmer Enspire 2300 for colorimetry and luminescence/AlphaLISA.  Absorbance filter sets; 405 nM; 450 nM; 570 nM; 650 nM
  3. BioRad Chemidoc XRS: Filter sets: Cy-3 630 BP30; Tex-Red 560DF50 for Q-Dot WB.
  4. µ-XRF (BrukerNano ARTAX-800). Up to 50 kV. Peltier cooled detector. X-ray spot size on target is 60 µm. Max Imaging area is 5 x 5 cm.
  5. LA-ICP-MS (Bruker M90 Aurora, and a LA by CETAC LSX-213 NdYAG). Laser spot size from 10 to 150 µm. Practical max imaging size of ca. 6 mm x 4 mm.
  6. Becton Dickinson LSR II Flow Cytometer
  7. Becton Dickinson FACSAria III Cell Sorter and Analyser

Image Analysis software and workstations

1. Linux workstation at DaMBIC:

Huygens for image deconvolution, ImageJ/Fiji, MATLAB, Icy for 3D image rendering, Ilastik for interactive image classification, 

2. Windows  workstation at DaMBIC:

  ImageJ/Fiji, VistaVision for for image acquisition, image processing and image display, ATLAB, Icy for 3D image rendering, LAS X for Leica images, CellProfiler software for quantitative analysis of biological images, FlimFit for FLIM adata analysis, 3D Slicer for image processing, and three-dimensional visualization, Microscopy Image Browser (MIB), Adobe Creative Cloud, JPK SPM Data Processing for AFM images

3.  Data Analysis Workstations at  Flow Cytometry Facility:

The LSR II and the FACSVantage/Diva cell-sorter employ PC-based DIVA software.  ata analysis workstations (PC and MAC), accessing FACSCORE shared Network server, are available for data processing with Cell Quest Pro software, DIVA-software and FLOWJo-software.

 

 

 

 

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