The aim of our studies is to provide insights into the role of protein oxidation in hepatocytes response to drugs, xenobiotics and genotoxic compounds.
We have developed a 3D cell culture-based model utilising hepatocarcinoma cell line and multicellular spheroids propagated in rotary bioreactors. We asses cell function by measuring physiological parameters such as glucose consumption, lipid uptake, lipid accumulation, ATP levels, protein content, glycogen levels and proliferation. We use proteomics to evaluate changes in protein expression and modification patterns and lipidomic analysis to follow lipid metabolism and accumulation. We have developed specific redox-proteomics approaches that allow us to follow S-nitrosylation and S-sulfenylation and protein carbonylation in biological samples.