Anastasiadous's Lab contributes to GALAXY WP2
The complex fine-tuning of cell function requires strict gene regulation operating in multiple levels. One of these is the post-transcriptional regulation with the application of microRNAs (miRNAs). miRNAs are small single strand RNAs that as part of the RNA-induced silencing complex (RISC), identify and suppress target-mRNAs, causing translational silencing. Through this mechanism the cell achieves gene regulation, not only in quantitative and temporal manner, but also local control at specific subcellular sites. Local responses and decentralized regulation can dictate signaling pathways that are involved in cell morphology, providing an important "framework" for cellular physiology. The focus of our research is to study the post-transcriptional gene regulation through the miRNA machinery and the RISC compounds, such as Argonauts. We aim to identify the expression profiles of miRNAs and their target-mRNAs that will enable us to characterize their interactions and create super-networks, aiming to dissect and deeply understand the molecular pathways involved in pathological conditions. Through our research we expect to tackle unmet medical needs by providing novel diagnostic, prognostic and therapeutic tools, leading to the successful management of multifactorial disease.