Professor Michael Kemp
Associate Professor Marianne N. Skov
Associate Professor Janne Kudsk Klitgaard
Professor Birgitte H. Kallipolitis
Enterococcus (E.) faecalis is the third most common cause of infectious endocarditis (IE) worldwide and is becoming increasingly prevalent, partly due to a progressive increase in the number of urogenital and abdominal procedures at the hospitals. Approximately 14% of all IE cases are caused by E. faecalis in Denmark and therefore we have investigated bacterial factors that can be associated with IE [Madsen et al., 2017)].
As such factors may include specific molecular types and genes encoding virulence factors we have also performed whole genome sequencing (WGS) of isolates of E. faecalis from patients with infective endocarditis or urogenital infections. In our ongoing studies, we aim to investigate how fatty acids may act to neutralize E. faecalis by inhibiting the growth and/or the production of key virulence factors. Presently, we are setting up methods in our laboratories for testing the expression of E. faecalis virulence factors (i.e. enzymatic assays, qPCR, Western Blot, Northern Blot) and genetic tools for constructing mutant variants of E. faecalis.
Read more about this project
Madsen K. T., Skov M. N., Gill S., Kemp M. (2017). Virulence factors associated with Enterococcus faecalis infective endocarditis: A mini review. Open Microbiol J. 11:1-11.