Professor Uffe Holmskov
Professor Boye L Jensen
Professor Peter Bie
Professor Jo De Mey
Professor mso Pernille B Lærkegaard Hansen
Professor Martin Tepel
Professor Ral Kôhler
Professor Søren Sheikh
Professor Lars Melholt Rasmussen
Docent emeritus Anthony M Carter
Docent Torben Johansen
Assoc. Prof. Grith Lykke Sørensen
Assoc. Prof. Ulla G Friis
Assoc. Prof. Ulrika Muscha Steckelings
Assoc. Prof. Jane Stubbe
Assistant Prof. Kirsten Madsen
Acad. Staff Anders Schlosser
- Renal and carsiovascular regulation of salt and water balance and blood pressure in the normal contition, during development, and under pathomogical salt and water re-taining conditions
- Cardiac stem cells
- Diabetes-associated vascular changes
- Receptor pharmacology and Cellular mechano-electrical transduction
Available methodologies, techniques and facilities
- Cell isolation and cell culture facilities for cell lines and primary cultures (juxtaglome-rular cells, vascular smooth muscle (renal, rat and human aortic), inner medullary collecting duct cells, mesangial cells, renin-producing AS4.1 cells, human aortic endothelial cells, rat cardiac myocytes and fibroblasts (neonatal and adult), and cardiac stem cells.
- Conscious dog and rat setup with servo mechanisms controlling body contents of water and salt and blood pressure.
- On line recordings of blood pressure in conscious unstressed mice
- Electrophysiological recordings on single cells (tight seal whole cell recordings): membrane potential (current clamp), current-voltage relationship, single channel (voltage clamp) and membrane capacitance.
- Using chamber with determination of transepithelial resistance and currentof epithelial monolayers.
- MicroRNA detection and visualization methods, including quantitative RT-PCR and in situ hybridization.
- Flow cytometry and cell sorting (FACS) of primary isolated cells (at the FACS Core facilities).
- Culturing and differentiation of murine embryonic stem cells.
- Quantification and profiling of cell numbers and cell sizes by use of Coulter counter (Z2).
- Mice models of cardiac diseases, including aortic stenosis and induction of cardiac infarctions.
- Enzyme activity measurements (nitric oxide synthase).
- Immunohistochemistry, immunohisto- and –cyto fluorescense for enzymes, ion transporters, proliferation, growth factors, hypoxia, and cell type specific markers..
- Intracellular pH and calcium concentration measurements by ratio fluorescence spectrometry and laser confocal imaging.
- Ion currents in isolated sensory epithelia by the voltage clamp technique.
- Measurement of metabolites (column chromatography; HPLC).
- Microdissection of blood vessels and single nephron segments
- Microperfusion of arteries and small blood vessels
- Recordings of isometric force in artery rings from rat, rabbit, mouse
- Molecular biology including isolation of RNA and DNA from cells and tissues, cDNA cloning, sequencing, RT-PCR, RNase protection assay, RNA differential display, Northern and Southern blotting, Western blotting, quantitative real-time PCR with SYBR green detection, transfection, site-directed mutagenesis, siRNA silencing.
- Osmotic pressure measurements.
- Plasma volume determination.
- Radioimmunoassays of vasoactive hormones, prostaglandins and cyclic nucleotides.
- Rat model for liver cirrhosis and nephrotic syndrome and adrenal insufficiency (adrenalectomy).
- Mouse trangenic breeding (eNOS-/-, COX-2-/-).
- Rat model for renal ischemia-reperfusion and infarct.
- Determination of urine concentration ability in rats and tissue interstitial osmolality.
- Receptor subtype determination.
- Renal Clearance techniques.
- Renin secretion from superfused and incubated juxtaglomerular cells.
- Determination of isometric force generation in arteries from mice, rats, and humans by myograph technique.
- Tension-measurements in smooth muscle.
- Uptake and release of transmitters determined by HPLC and liquid scintillation spectrometry.
- Available methodologies, techniques and facilities