Forskningsområder

Forskningsområder:

Modeller for cerebral iskæmi, herunder global og fokal cerebral iskæmi, rygmarvslæsion og funktionelle adfærdsmodeller i mus.

Udvalgsarbejde:

Bestyrelsesmedlem i Dansk Selskab for Neurovidenskab 2007-2011

Selekterede foredrag:

"Protective neuroinflammation in ischemic-induced neurodegeneration". Pharmacology, Anatomical Neuropharmacology and Drug Discovery Seminars for Mechaelmas Term 2011, University of Oxford, UK, November 29th, 2011.

"Significance of microglial tumor necrosis factor signaling for ischemic brain damage". Graduate Program in Areas of Basic and Applied Biology (GABBA), Institute for Molecular and Cell Biology, Porto, Portugal, June 2, 2011.

"Stroke and inflammation". Graduate Program in Areas of Basic and Applied Biology (GABBA), Institute for Molecular and Cell Biology, Porto, Portugal, May 3, 2011.

"Protective neuroinflammation in ischemic-induced neurodegeneration", Institut for Medicinsk Biokemi, Århus Universitet, Århus, Denmark, May 10, 2011.

"Protective neuroinflammation in ischemic-induced neurodegeneration", Institute for Molecular and Cell Biology, Porto, Portugal, September 3, 2010.

"Rygmarvsskader og Stamcelleterapi", Sundheds og Forskningsdagen, RYK og PTU, Kolding, September 24, 2009.

"Microglial tumor necrosis factor: Mediator of neuronal life and death ?", Miami Project Lecture Series, The Miami Project to Cure Paralysis. University of Miami Miller School of Medicine, Miami, FL, April 29, 2009.

Lambertsen gruppen:

* Phd-studerende Ditte Gry Ellman (barselsorlov marts 2012 - )
* Specialestud. Mithula Sivasaravanaparan
* Specialestud. Torben Fogtmann
* Specialestud. Maria Gammelstrup Andersen (samarbejdsprojekt med Kristian Strømgaard, KU)
* Specialestud. Nellie Anne Martin (udvekslingsstudent hos Jochen Prehn, Royal College of Surgeons in Ireland)
* ITEK stud. Maria Ormhøj
* ITEK stud. Pernille Madsen
* Skolarstipendiat Hans Gram Novrup
* Bachelorstud. Stine Thyssen
* Bachelorstud. Leena Karimi
* Bachelorstud. Miriam Mohamed
* Bachelorstud. Cansu Karabiyik
* Bachelorstud. Lujitha suntharalingam
* Laborant Karen Rich (barselsorlov februar 2012 - )
* Laborant Louise Lykkemark Christensen (barselsvikariat 2012 - )

 Publikationer: 

Akademiske afhandlinger:
1. Lambertsen KL (1999) The role of interferon-gamma (IFNγ) and tumor necrosis factor (TNF) in focal cerebral ischemia in mice. Specialerapport, Det Naturvidenskabelige Fakultet, Syddansk Universitet.

2. Lambertsen KL (2004) The patophysiological role of tumor necrosis factor and interferon gamma in focal cerebral ischemia in mice. PhD-afhandling, Det Sundhedsvidenskabelige Fakultet, Syddansk Universitet.


Artikler:
1. Gregersen R, Lambertsen K, and Finsen B (2000) Microglia and macrophages are the major source of tumor necrosis factor (TNF) in permanent middle cerebral artery occlusion in mice. J Cereb Blood Flow Metab, 20:53-65.

2. Lambertsen KL, Gregersen R, Drøjdahl N, Owens T and Finsen B (2001) A specific and sensitive method for visualization of tumor necrosis factor in the murine central nervous system. Brain Res Brain Res Protocols 7:175-191.

3. Lambertsen KL, Gregersen R, and Finsen B (2002) Microglial-macrophage synthesis of tumor necrosis factor after focal cerebral ischemia in mice is strain dependent. J Cereb Blood Flow Metab 22:785-797.

4. Lambertsen KL, Gregersen R, Meldgaard M, Clausen BH, Heibøl EK, Ladeby R, Knudsen J, Frandsen A, Owens T, Finsen B (2004) A role for interferon-gamma in focal cerebral ischemia in mice. J Neuropathol Exp Neurol 63:942-955.

5. Gramsbergen JB, Skjøth-Rasmussen J, Rasmussen C, Lambertsen KL (2004) On-line monitoring of striatum glucose and lactate in the endothelin-1 rat model of transient, focal cerebral ischemia using microdialysis and flow-injection analysis with biosensors. J Neurosci Methods 140:93-101.

6. Lambertsen KL (2004) Tumor nekrose faktors og interferon-gammas patofysiologiske rolle ved fokal cerebral iskaemi i mus. Ugeskrift for Laeger 166:3011.

7. Lambertsen KL, Meldgaard M, Ladeby R, Finsen B (2005) A quantitative study of microglial-macrophage synthesis of tumor necrosis factor during acute and late focal cerebral ischemia. J Cereb Blood Flow Metab 25:119-136.

8. Clausen BH, Lambertsen KL, Meldgaard M, Finsen B (2005) A quantitative in situ hybridization and polymerase chain reaction study of microglial-macrophage expression of interleukin-1β mRNA following permanent middle cerebral artery occlusion in mice. Neuroscience 132:879-892.

9. Wirenfeldt M, Babckock AA, Ladeby R, Lambertsen KL, Dagnaes-Hansen F, Leslie G, Owens T, Finsen B (2005) Microglial subpopulations in the acutely injured central nervous system. J Neurosci Res 82:507-512.

10. Lyck L, Jelsing J, Jensen PS, Lambertsen KL, Pakkenberg B, Finsen B (2005) Immunohistochemical visualization of neurons and specific glial cells for stereological application in the porcine neocortex. J Neurosci Methods 152:229-242.

11. Fenger C, Drøjdahl N, Wirenfeldt M, Sylvest L, Jørgensen OS, Meldgaard M, Lambertsen KL, Finsen B (2006) Tumor necrosis factor and its p55 and p75 receptors are not required for axonal lesion-induced microgliosis in mouse fascia dentata. Glia 54:591-605.

12. Meldgaard M, Fenger C, Lambertsen KL, Pedersen MD, Finsen B. (2006) Validation of two reference genes for mRNA level studies of murine disease models in neurobiology. J Neurosci Methods 156:101-110.

13. Clausen BH, Lambertsen KL, Finsen B (2006) Glyceraldehyde-3-phosphate dehydrogenase versus toluidine blue as a marker for infarct volume estimation following permanent occlusion of the middle cerebral artery in mice. Exp Brain Res 175:60-67.

14. Lambertsen KL, Clausen BH, Fenger C, Wulf H, Owens T, Dagnæs-Hansen F, Meldgaard M, Finsen B (2007) Microglia and macrophages express TNF receptor p75 following middle cerebral artery occlusion in mice. Neuroscience 144:934-949.

15. Degn M, Lambertsen KL, Petersen G, Meldgaard M, Artmann A, Clausen BH, Hansen SH, Finsen B, Hansen HS, Lund TM. Changes in brain levels of N-acetylethanolamines and 2-arachidonoyl glycerol in focal cerebral ischemia in mice. J Neurochem 103:1907-1916.

16. Clausen BH, Lambertsen KL, Babcock A, Holm TH, Dagnæs-Hansen F, Finsen B (2008) Interleukin-1β and tumor necrosis factor-α are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflam, 5:46. (Delt førsteforfatterskab).

17. Nielsen M, Lambertsen KL, Clausen BH, Meldgaard M, Diemer NH, Zimmer J, Finsen B (2009) Nuclear translocation of endonuclease G in degenerating neurons after permanent middle cerebral artery occlusion in mice. Exp Brain Res, 194:17-27.

18. Lambertsen KL, Clausen BH, Babcock AA, Gregersen R, Nielsen M, Wirenfeldt M, Haugaard LS, Færgemann NJ, Dagnaes-Hansen F, Bluethmann H, Meldgaard M, Deierborg T, Finsen B (2009) Microglia protect neurons against ischemia by synthesis of tumor necrosis factor. J Neurosci, 29:1319-1330.

19. Santos SD, Lambertsen KL, Clausen BH, Akinc A, Alvarez R, Finsen B, Saraiva MJ (2010) Cerebrospinal fluid transthyretin neuroprotection in a mouse model of brain ischemia. J Neurochem, 115:1434-1444.

20.Lambertsen KL, Deierborg T, Gregersen R, Clausen BH, Wirenfeldt M, Nielsen HH, Dalmau I, Diemer NH, Dagnaes-Hansen F, Johansen FF, Keating A, Finsen B (2011) Differences in origin of reactive microglia in bone marrow chimeric mouse and rat after transient global ischemia. J Neuropathol Exp Neurol 70:481-494.

21.Nielsen HH, Toft-Hansen H, Lambertsen KL, Owens T, Finsen B (2011) Promotion of oligodendrocyte formation by myelin reactive T-cells. Am J Pathol 179:2028-2041

22. Bach A, Clausen BH, Vestergaard B, Chi CN, Round A, Sørensen PL, Nissen KB, Kastrup JS, Gajhede M, Jemth P, Kristensen AS, Lundström P, Lambertsen KL, Strømgaard K (2012) High-affinity, dimeric inhibitors of PSD-95 efficiently protect against ischemic brain damage. Proc Natl Acad Sci USA 109:3317-3322.

23. Lambertsen KL, Biber K, Finsen B (2012) Inflammatory cytokines in experimental and human stroke. J Cereb Blood Flow Metab, in press (invited review).

24.Brambilla R, Couch Y, Lambertsen KL (2012) The effect of stroke on immune funtion. Molecular and Cellular Neuroscience, submitted (invited review).

Bogkapitler:
1. Skjøth-Rasmussen J, Lambertsen K, Gramsbergen JB (2003) On-line glucose and lactate monitoring in rat striatum: Effect of malonate and correlation with histological damage. In: Monitoring Molecules in Neuroscience. Proceedings of the 10th International Conference on In Vivo Methods. Eds. J. Kehr, K. Fuxe, U. Ungerstedt, T.H. Svensson, Karolinska Institutet, Stockholm, pp. 265-267.

2. Lambertsen KL, Deierborg T (2012) A detrimental role of MIF in ischemic brain damage. In: The MIF Handbook, World Scientific Publishing Co. Editor: Richard Bucala,Yale University, in press.

Internationale og nationale samarbejdspartnere:

1. Dr. Tomas Deierborg, Department of Experimental Medical Science, University of Lund.

2. Dr. Daniel Anthony, Department of Pharmacology, University of Oxford.

3. Dr. Sofia Duque Santos, Institute for Molecular and Cell Biology, University of Porto.

4. Dr. Roberta Brambilla, The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine.

5. Dr. John Bethea and Valerie Bracchi-Ricard, The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine.

6. Dr. Mette Berendt, LIFE, University of Copenhagen

7. Dr. Karin Lykke-Hartmann, Department of Biomedicine, University of Aarhus

8. Dr. Kristian Strømgaard, Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen

9. Dr. David Szymkowski, Xencor Inc, Monrovia, CA

Arbejdsopgaver

Lektor

Underviser i:

Bachelor i Logopædi og teknisk og pædagogisk Audiologi, Anatomi og Fysiologi.

SU501 Anatomi for Biomedicinere.

Human Patofysiologi for Biomedicinere, Delkursus i Centralnervesystemets Sygdomme

CV

Lektor, Institut for Molekylær Medicin, Neurobiologisk Forskning, Syddansk Universitet, 2009-

Udenlandsophold, Dr. John Bethea, The Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, 2008-2009.                                                                                                                                                     

Forskningsadjunkt, Medicinsk Bioteknologisk Center, Syddansk Universitet, 2005-2009.

Postdoc, Anatomi og Neurobiologi, Syddansk Universitet, 2004.

PhD i Sundhedsvidenskab, Anatomi og Neurobiologi, Syddansk Universitet, 2000-2004.

Cand scient i Biomedicin, Anatomi og Neurobiologi, Syddansk Universitet, 1997-1999.

Bachelor i Molekylærbiologi, Patologisk Institut, Syddansk Universitet, 1996.

Publikationer

Aktiviteter